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Anti-NSE/ENO2 Antibody (3T60) is a Mouse antibody targeting NSE/ENO2. Anti-NSE/ENO2 Antibody (3T60) can be used in WB.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $199 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: WB Reactivity: Human |
Alias | NSE, HEL-S-279, enolase 2 (γ, neuronal), enolase 2 (gamma, neuronal) |
Ig Type | Mouse IgG1 |
Clone | 3T60 |
Reactivity | Human |
Verified Activity | Anti-ENO2 mouse monoclonal antibody at 1:200 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Hela Whole Cell Lysate. -Lane C: Jurkat Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:49 kDa |
Application | WB |
Recommended Dose | WB: 1:500-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human NSE/ENO2 (rh NSE/ENO2; TMPY-02646; P09104; Met1-Leu434). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | The combination of silencing ENO2 and 2-deoxyglucose (2-DG) synergistically inhibited leukemia cell survival. ENO2 may be a biological marker for monitoring chemotherapeutic efficacy and relapse in ALL. Reduced ENO2 expression may be a biomarker for a subset of autistic children. Neuron specific enolase (ENO2, gamma-enolase) has been used as a biomarker to help identify neuroendocrine differentiation in breast cancer. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human NSE/ENO2 Protein (TMPY-02646) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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