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Anti-NSE Antibody (5G373)
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Catalog No. TMAB-09646
Anti-NSE Antibody (5G373) is a Rabbit antibody targeting NSE. Anti-NSE Antibody (5G373) can be used in WB,IF,IHC-P,IHC-Fr.
Anti-NSE Antibody (5G373)
Anti-NSE Antibody (5G373)
Copy Product Info😃Good
Catalog No. TMAB-09646
Anti-NSE Antibody (5G373) is a Rabbit antibody targeting NSE. Anti-NSE Antibody (5G373) can be used in WB,IF,IHC-P,IHC-Fr.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $148 | 7-10 days | 7-10 days | |
| 50 μL | $264 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-NSE Antibody (5G373) is a Rabbit antibody targeting NSE. Anti-NSE Antibody (5G373) can be used in WB,IF,IHC-P,IHC-Fr. |
| Ig Type | IgG |
| Clone | 5G373 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NSE Monoclonal Antibody, Unconjugated (TMAB-09646) at 1: 200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. 9. 25 μg total protein per lane of various lysates (see on figure) probed with NSE monoclonal antibody, unconjugated (TMAB-09646) at 1:3000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IF: 1:50-200; IHC-P: 1:50-200; IHC-Fr: 1:50-200 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. |
| Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | PBS, Glycerol, BSA. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]. |
| Immunogen | A synthesized peptide: human gamma Enolase |
| Antigen Species | Human |
| Gene Name | ENO2 |
| Gene ID | |
| Protein Name | gamma-enolase |
| Uniprot ID | |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
| Molecular Weight | Theoretical: 48 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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