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Anti-NSDHL Antibody (6E747)

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Catalog No. TMAB-09643

Anti-NSDHL Antibody (6E747) is a Rabbit antibody targeting NSDHL. Anti-NSDHL Antibody (6E747) can be used in FCM, IF, IHC-Fr, IHC-P, WB.

Anti-NSDHL Antibody (6E747)

Anti-NSDHL Antibody (6E747)

Copy Product Info
😃Good
Catalog No. TMAB-09643
Anti-NSDHL Antibody (6E747) is a Rabbit antibody targeting NSDHL. Anti-NSDHL Antibody (6E747) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1497-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NSDHL Antibody (6E747) is a Rabbit antibody targeting NSDHL. Anti-NSDHL Antibody (6E747) can be used in FCM, IF, IHC-Fr, IHC-P, WB.
Ig Type
IgG
Clone
6E747
Reactivity
Human
Verified Activity
1. The PANC-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NSDHL antibody (TMAB-09643, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 ug total protein per lane of various lysates (see on figure) probed with NSDHL monoclonal antibody, unconjugated (TMAB-09643) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
Application
Recommended Dose
FCM=1:50-100; IF=1:50-200; IHC-Fr=1:100-200; IHC-P=1:100-200; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass membrane protein.
Tissue SpecificityBrain, heart, liver, lung, kidney, skin and placenta.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundCatalyzes the NAD(P)+-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity).Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).
Antigen Details
Immunogen
A synthesized peptide: human NSDHL
Antigen Species
Human
Gene Name
NSDHL
Gene ID
Protein Name
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 42 kDa. Actual: 38 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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