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Anti-NSBP1 Polyclonal Antibody

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Catalog No. TMAB-09641

Anti-NSBP1 Polyclonal Antibody is a Rabbit antibody targeting NSBP1. Anti-NSBP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-NSBP1 Polyclonal Antibody

Anti-NSBP1 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-09641
Anti-NSBP1 Polyclonal Antibody is a Rabbit antibody targeting NSBP1. Anti-NSBP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5287-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NSBP1 Polyclonal Antibody is a Rabbit antibody targeting NSBP1. Anti-NSBP1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NSBP1 Polyclonal Antibody, Unconjugated (TMAB-09641) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Paraformaldehyde-fixed, paraffin embedded (human testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NSBP1) Polyclonal Antibody, Unconjugated (TMAB-09641) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Note=Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin (By similarity).
Tissue SpecificityUbiquitously expressed.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundNSBP1 binds specifically to nuclear core particles and acts as a transcriptional activator. [Subcellular Location] Nucleus. [Tissue Specificity] Widely expressed with highest levels in submaxillary gland, thymus, kidney and liver and lowest levels in brain, lung, pancreas and eye. [Developmental Stage] Highest levels are found in 7-day-old embryos. Levels in the 7-day-old embryo are 4-fold higher than in the adult and almost 10-fold higher than in later embryonic stages. [SIMILARITY] Belongs to the HMGN family.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NSBP1
Antigen Species
Human
Gene Name
HMGN5
Gene ID
Protein Name
High mobility group nucleosome-binding domain-containing protein 5
Uniprot ID
Function
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction (By similarity).
Chemical Properties
Molecular WeightTheoretical: 32 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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