Anti-NRSF Polyclonal Antibody is a Rabbit antibody targeting NRSF. Anti-NRSF Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Horse,Rabbit)

1. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-NRSF (TMAB-01273) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 121 kDa
2. Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated (TMAB-01273) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. NRSF was immunoprecipitated from human hela cells lysate with TMAB-01273 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: TMAB-01273 IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All
Lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
4. Sample:
Hela (Human) Cell Lysate at 30 μg
Hela KO NRSF (Human) Cell Lysate at 30 μg
Primary: Anti-NRSF (TMAB-01273) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 121 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NRSF

Human

REST

RE1-silencing transcription factor

Co-factors,Zinc Finger,Neurogenesis

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.