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Anti-NRSF Polyclonal Antibody

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Catalog No. TMAB-01273

Anti-NRSF Polyclonal Antibody is a Rabbit antibody targeting NRSF. Anti-NRSF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NRSF Polyclonal Antibody

Anti-NRSF Polyclonal Antibody

😃Good
Catalog No. TMAB-01273
Anti-NRSF Polyclonal Antibody is a Rabbit antibody targeting NRSF. Anti-NRSF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$372 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NRSF Polyclonal Antibody is a Rabbit antibody targeting NRSF. Anti-NRSF Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
Raji (Human) Cell Lysate at 30 μg
Primary: Anti-NRSF (TMAB-01273) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 121 kDa
2. Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated (TMAB-01273) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. NRSF was immunoprecipitated from human hela cells lysate with TMAB-01273 at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: TMAB-01273 IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All
Lanes: Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
4. Sample:
Hela (Human) Cell Lysate at 30 μg
Hela KO NRSF (Human) Cell Lysate at 30 μg
Primary: Anti-NRSF (TMAB-01273) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kDa
Observed band size: 121 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NRSF
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Co-factors,Zinc Finger,Neurogenesis
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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