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Anti-NRROS Polyclonal Antibody

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Catalog No. TMAB-01272
Alias Transforming growth factor beta activator LRRC33, NRROS, Negative regulator of reactive oxygen species, LRRC33, Leucine-rich repeat-containing protein 33

Anti-NRROS Polyclonal Antibody is a Rabbit antibody targeting NRROS. Anti-NRROS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NRROS Polyclonal Antibody

Anti-NRROS Polyclonal Antibody

😃Good
Catalog No. TMAB-01272Alias Transforming growth factor beta activator LRRC33, NRROS, Negative regulator of reactive oxygen species, LRRC33, Leucine-rich repeat-containing protein 33
Anti-NRROS Polyclonal Antibody is a Rabbit antibody targeting NRROS. Anti-NRROS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NRROS Polyclonal Antibody is a Rabbit antibody targeting NRROS. Anti-NRROS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
AliasTransforming growth factor beta activator LRRC33, NRROS, Negative regulator of reactive oxygen species, LRRC33, Leucine-rich repeat-containing protein 33
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Rabbit)
Verified Activity
1. Sample:
Bone (Rat) Cell Lysate at 40 μg
Thymus (Rat) Cell Lysate at 40 μg
Primary: Anti-LRRC33 (TMAB-01272) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (TMAB-01272) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LRRC33) Polyclonal Antibody, Unconjugated (TMAB-01272) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nucleus staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single pass type I membrane protein.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundLRRC33 is a 692 amino acid protein that contains 17 LRR repeats. The gene encoding LRRC33 maps to chromosome 3, which encodes over 1,100 genes. Particular regions of the chromosome 3 short arm are deleted in many types of cancer cells. Marfan syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth disease are a few of the numerous genetic diseases associated with chromosome 3.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LRRC33
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 74 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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