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Anti-NRF1 Antibody (4L389)

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Catalog No. TMAB-09635

Anti-NRF1 Antibody (4L389) is a Mouse antibody targeting NRF1. Anti-NRF1 Antibody (4L389) can be used in WB,ICC/IF.

Anti-NRF1 Antibody (4L389)

Anti-NRF1 Antibody (4L389)

Copy Product Info
😃Good
Catalog No. TMAB-09635
Anti-NRF1 Antibody (4L389) is a Mouse antibody targeting NRF1. Anti-NRF1 Antibody (4L389) can be used in WB,ICC/IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1517-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4737-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NRF1 Antibody (4L389) is a Mouse antibody targeting NRF1. Anti-NRF1 Antibody (4L389) can be used in WB,ICC/IF.
Ig Type
IgG2b/Kappa
Clone
4L389
Reactivity
Human
Verified Activity
1. Cell line: HepG2
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary Ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary Ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Counter stain: Tubulin (Red)
Comment: Color green is the positive signal for TMAB-09635
2. Sample:
Lane 1: Human Hela cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human 293T cell lysates
Lane 5: Human Jurkat cell lysates
Primary: Anti-NRF1 (TMAB-09635) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Application
Recommended Dose
WB: 1:500-2000; ICC/IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus
Tissue SpecificityUbiquitously expressed with strongest expression in skeletal muscle.
ConstructionRecombinant Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NRF1
Antigen Species
Human
Gene Name
NRF1
Gene ID
Protein Name
Nuclear respiratory factor 1
Uniprot ID
Function
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Chemical Properties
Molecular WeightTheoretical: 55 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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