Anti-NRBP2 Antibody (3S983) is a Mouse antibody targeting NRBP2. Anti-NRBP2 Antibody (3S983) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG2a, Kappa

3S983

Human (predicted:Mouse)

1. Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate:1: HeLa, 2: SH-SY5Y, 3: Mouse brain
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 58 kDa
Observed MW: 58 kDa
2. Cell line: HEK-293
Fixative: 4% Paraformaldehyde
Permeabilization: 0.1% TritonX-100
Primary ab dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary ab: Goat Anti-Mouse IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-09632
3. Tissue: Human colon cancer
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:100
Primary ab incubation condition: 1 hour at room temperature
Secondary ab: SP Kit (Mouse)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-09632

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500

Monoclonal

NRBP2

Nuclear receptor-binding protein 2

May regulate apoptosis of neural progenitor cells during their differentiation.