Anti-NRAS Polyclonal Antibody is a Rabbit antibody targeting NRAS. Anti-NRAS Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Pig,Cow)

1. Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated (TMAB-09628) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-N-Ras Polyclonal Antibody, Unconjugated (TMAB-09628) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated used at 1: 200 dilution for 40 minutes at 37°C.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human N-Ras

Human

NRAS

GTPase NRas

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.