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Anti-NRAS Antibody (8O77)
😃Good
Catalog No. TMAC-03537
Alias NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4
Anti-NRAS Antibody (8O77) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (8O77) can be used in WB,IP,FCM.
Anti-NRAS Antibody (8O77)
Anti-NRAS Antibody (8O77)
😃Good
Catalog No. TMAC-03537Alias NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4
Anti-NRAS Antibody (8O77) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (8O77) can be used in WB,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-NRAS Antibody (8O77) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (8O77) can be used in WB,IP,FCM. |
| Synonyms | NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7, Lane 2: 293T, Lane 3: Mouse brain. 2. Flow cytometric analysis of Hela cells with Ras antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; FCM: 1:10-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 18 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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