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Anti-NRAS Antibody (5T588)
😃Good
Catalog No. TMAC-03538
Alias NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4
Anti-NRAS Antibody (5T588) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (5T588) can be used in ICC/IF,IP,WB.
Anti-NRAS Antibody (5T588)
Anti-NRAS Antibody (5T588)
😃Good
Catalog No. TMAC-03538Alias NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4
Anti-NRAS Antibody (5T588) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (5T588) can be used in ICC/IF,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-NRAS Antibody (5T588) is a Rabbit antibody targeting NRAS. Anti-NRAS Antibody (5T588) can be used in ICC/IF,IP,WB. |
| Synonyms | NS6, NRAS1, N-ras, neuroblastoma RAS viral (v-ras) oncogene homolog, NCMS, CMNS, ALPS4 |
| Ig Type | IgG |
| Clone | 5T588 |
| Reactivity | Human,Mouse,Rat,zebrafish |
| Verified Activity | 1. Western blot analysis of Ras on different lysates using anti-Ras antibody at 1/1,000 dilution. Positive control: Lane 1: 293T, Lane 2: MCF-7, Lane 3: Hela, Lane 4: zebrafish. 2. ICC staining Ras in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 3. ICC staining Ras in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining Ras in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; ICC/IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 21 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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