Anti-NRAP Polyclonal Antibody is a Rabbit antibody targeting NRAP. Anti-NRAP Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human

1. Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NRAP Polyclonal Antibody, Unconjugated (TMAB-09627) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NRAP Polyclonal Antibody, Unconjugated (TMAB-09627) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NRAP

Human

NRAP

Nebulin-related-anchoring protein

May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.