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Anti-NR3C1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01266
Alias nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor), GRL, GR, GCRST, GCR, GCCR

Anti-NR3C1 Polyclonal Antibody is a Rabbit antibody targeting NR3C1. Anti-NR3C1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-NR3C1 Polyclonal Antibody

Anti-NR3C1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01266Alias nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor), GRL, GR, GCRST, GCR, GCCR
Anti-NR3C1 Polyclonal Antibody is a Rabbit antibody targeting NR3C1. Anti-NR3C1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-NR3C1 Polyclonal Antibody is a Rabbit antibody targeting NR3C1. Anti-NR3C1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Aliasnuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor), GRL, GR, GCRST, GCR, GCCR
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucocorticoid receptor) Polyclonal Antibody, Unconjugated (TMAB-01266) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Cell: H-4-II-E
Concentration:1:100
Host/Isotype: Rabbit/IgG
Flow cytometric analysis of primary antibody (Cat#: TMAB-01266) on H-4-II-e (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GCR) Polyclonal Antibody, Unconjugated (TMAB-01266) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Blank control: A549. Primary Antibody (green line): Rabbit Anti-Glucocorticoid receptor antibody (TMAB-01266)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Glucocorticoid receptor) Polyclonal Antibody, Unconjugated (TMAB-01266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand, nuclear after ligand-binding. Isoform Beta: Nucleus. Note=Localized largely in the nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSteroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Glucocorticoid receptor
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Nuclear hormone receptors,Corticoid,Corticoid
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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