Anti-NR2C2 Antibody (8J526) is a Rabbit antibody targeting NR2C2. Anti-NR2C2 Antibody (8J526) can be used in WB,FCM.

IgG

8J526

Human (predicted:Mouse,Rat)

1. The Jurkat (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NR2C2 antibody (TMAB-09614, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with NR2C2 monoclonal antibody, unconjugated (TMAB-09614) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-2000; FCM: 1:50-100

Monoclonal

A synthesized peptide: human NR2C2

Human

NR2C2

Nuclear receptor subfamily 2 group C member 2

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.