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Anti-NR0B2 Polyclonal Antibody

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Catalog No. TMAB-01262

Anti-NR0B2 Polyclonal Antibody is a Rabbit antibody targeting NR0B2. Anti-NR0B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NR0B2 Polyclonal Antibody

Anti-NR0B2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01262
Anti-NR0B2 Polyclonal Antibody is a Rabbit antibody targeting NR0B2. Anti-NR0B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3747-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NR0B2 Polyclonal Antibody is a Rabbit antibody targeting NR0B2. Anti-NR0B2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NROB2 Polyclonal Antibody, Unconjugated (TMAB-01262) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (TMAB-01262) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NR0B2) Polyclonal Antibody, Unconjugated (TMAB-01262) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Rat Kidney tissue lysates
Lane 2: Rat Small intestine tissue lysates
Primary: Anti-NR0B2 (TMAB-01262) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Cytoplasm. Note=Colocalizes with NEUROD1 in the nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundSHP is an orphan nuclear receptor containing the dimerization and ligand-binding domains found in other nuclear receptors, but lacking the conserved DNA binding domain. SHP is specifically expressed in liver and other tissues, including fetal liver and adrenal gland, as well as adult spleen and small intestine. In addition, SHP is highy expressed in the murine macrophage cell line RAW 264.7 but suppressed by oxLDL and 13-HODE, which is a ligand for PPARg. SHP interacts with nuclear receptors, including thyroid receptor, retinoic acid receptors (RAR and RXR) and estrogen receptors (ERa and ERb). SHP functions as a negative regulator of these receptors by at least three mechanisms: inhibition of DNA binding via dimerization, direct antagonism of coactivator function through competition and possibly transrepression via recruitment of putative corepressors. In oxLDL-treated, resting macrophage cells, SHP acts as a transcription coactivator of NFkB, suggesting that SHP is a modulatory component in the regulation of the transcriptional activities of NFkB. Lastly, negative feedback regulation of a hepatic bile acid transporter, NTCP, is controlled by bile acid-activated FXR via induction of SHP to protect the hepatocyte from bile acid-mediated damage in cholestatic conditions.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NR0B2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Retinoic & Retinoid,Lipid metabolism,Retinoic & Retinoid
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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