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Anti-NPY2R Polyclonal Antibody
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Catalog No. TMAB-01260
Anti-NPY2R Polyclonal Antibody is a Rabbit antibody targeting NPY2R. Anti-NPY2R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-NPY2R Polyclonal Antibody
Anti-NPY2R Polyclonal Antibody
😃Good
Catalog No. TMAB-01260
Anti-NPY2R Polyclonal Antibody is a Rabbit antibody targeting NPY2R. Anti-NPY2R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-NPY2R Polyclonal Antibody is a Rabbit antibody targeting NPY2R. Anti-NPY2R Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-NPY2R Polyclonal Antibody, Unconjugated (TMAB-01260) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: A549 Cell Lysate at 30 μg Primary: Anti-NPY2R (TMAB-01260) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 43 kDa 3. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-NPY2R (TMAB-01260) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kDa Observed band size: 45 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Neuropeptide Y (NPY), a peptide widely expressed in the brain, acts through the protein G-coupled receptors Y1, Y2 and Y5. This peptide modulates many important functions such as the control of energy balance and anxiety. Its involvement in brain development has been less investigated. Neuropeptide Y (NPY) receptors are members of a G protein coupled receptor superfamily and they mediate a variety of physiological responses including feeding and vasoconstriction. The endogenous ligands of NPY receptors are a family of structurally related peptides, including NPY, peptide YY (PYY), and pancreatic polypeptide (PP). Increased activity of NPY and its receptors, Y1R, Y2R, and Y5R has been found in the brain in many forms of experimental obesity. Furthermore, several association studies have supported the association between NPY2R and obesity. |
| Immunogen | KLH conjugated synthetic peptide: human NPY2R |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Angiogenic Factors,Diabetes associated,Lipoprotein metabolism,Lipoprotein metabolism,Cancer,Diabetes,Heart disease,Receptors,More GPCR |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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