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Anti-NPHS2 Antibody (4M580)

Catalog No. TMAB-09590 Copy Product Info
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Anti-NPHS2 Antibody (4M580) is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Antibody (4M580) can be used in IF, IHC-Fr, IHC-P.

Anti-NPHS2 Antibody (4M580)

Copy Product Info
🥰Excellent
Catalog No. TMAB-09590

Anti-NPHS2 Antibody (4M580) is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Antibody (4M580) can be used in IF, IHC-Fr, IHC-P.

Anti-NPHS2 Antibody
(4M580)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-NPHS2 Antibody (4M580) is a Rabbit antibody targeting NPHS2. Anti-NPHS2 Antibody (4M580) can be used in IF, IHC-Fr, IHC-P.
Ig Type
IgG
Clone
4M580
Reactivity
Human,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated (TMAB-09590) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NPHS2 Monoclonal Antibody, Unconjugated (TMAB-09590) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with NPHS2 Monoclonal Antibody, Unconjugated (TMAB-09590) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
Application
Recommended Dose
IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane; Peripheral membrane protein.
Tissue SpecificityAlmost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Concentration1mg/ml
Research BackgroundPodocin is an important member of a group of proteins shown to be associated with the slit diaphragm. Podocin belongs to the band 7 stomatin family of lipid raft-associated proteins. It is a hairpin like integral membrane protein with intracellular N and C termini. Podocin is located at the insertion site of the slit membrane, and is thought to act as a scaffold protein required to maintain or regulate the structural integrity of the slit diaphragm. It plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Antigen Details
Immunogen
A synthesized peptide: human Podocin
Antigen Species
Human
Gene Name
NPHS2
Gene ID
Protein Name
Podocin
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 42 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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