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Anti-NPFFR1 Polyclonal Antibody

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Catalog No. TMAB-01257

Anti-NPFFR1 Polyclonal Antibody is a Rabbit antibody targeting NPFFR1. Anti-NPFFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NPFFR1 Polyclonal Antibody

Anti-NPFFR1 Polyclonal Antibody

😃Good
Catalog No. TMAB-01257
Anti-NPFFR1 Polyclonal Antibody is a Rabbit antibody targeting NPFFR1. Anti-NPFFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$373 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-NPFFR1 Polyclonal Antibody is a Rabbit antibody targeting NPFFR1. Anti-NPFFR1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (TMAB-01257) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
U251 (Human) Cell Lysate at 30 μg
Primary: Anti-NPFF1 Receptor (TMAB-01257) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NPFF1 Receptor) Polyclonal Antibody, Unconjugated (TMAB-01257) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Cerebellum tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Cerebellum tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Primary: Anti-NPFF1 Receptor (TMAB-01257) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNeuropeptide FF 1 Receptor (NPFF1 or hFF1) and Neuropeptide FF 2 Receptor (NPFF2) belong to the G protein-coupled receptor 1 family. Both NPFF1 and NPFF2 are integral membrane proteins that act as receptors for NPAF (A-18- F-amide) and NPFF (F-8-F-amide) neuropeptides. Both NPFF proteins may be activated by synthetic or naturally occurring FMRF-amide-like ligands. The receptors are mediated by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. NPFF1 Receptors is highly expressed in the human hypothalamus and amygdala, indicating a possible role for NPFF1 in central autonomic and neuroendocrine control in the human brain. Based in part on NPFF2 Receptor expression in diencephalon and superficial layers of the spinal cord, NPFF2 Receptor is thought to be involved in the modulation of sensory input and opioid analgesia.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GPR147/NPFF1 Receptor
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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