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Anti-NONO Antibody (8D270) (Synonyms: p54nrb, NRB54, Non-POU domain-containing octamer-binding protein, NonO ...)
Catalog No. TMAC-03002 Copy Product Info
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Anti-NONO Antibody (8D270) is a Rabbit antibody targeting NONO. Anti-NONO Antibody (8D270) can be used in ICC,IF,IHC,WB.
Anti-NONO Antibody (8D270)
Copy Product Info🥰Excellent
Catalog No. TMAC-03002
Synonyms p54nrb, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, NMT55, DNA-binding p52/p100 complex, 55 kDa nuclear protein, 54 kDa nuclear RNA- and DNA-binding protein, 52 kDa subunit
Anti-NONO Antibody (8D270) is a Rabbit antibody targeting NONO. Anti-NONO Antibody (8D270) can be used in ICC,IF,IHC,WB.
Anti-NONO Antibody
(8D270)
(8D270)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NONO Antibody (8D270) is a Rabbit antibody targeting NONO. Anti-NONO Antibody (8D270) can be used in ICC,IF,IHC,WB. |
| Synonyms | p54nrb, NRB54, Non-POU domain-containing octamer-binding protein, NonO protein, NMT55, DNA-binding p52/p100 complex, 55 kDa nuclear protein, 54 kDa nuclear RNA- and DNA-binding protein, 52 kDa subunit |
| Ig Type | IgG |
| Clone | 8D270 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of p54nrb on 293 (1) and SH-SY-5Y (2) cell lysate using anti-p54nrb antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-p54nrb antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- p54nrb antibody. Counter stained with hematoxylin. 7. ICC staining p54nrb in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.        |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Found in both primary and transformed human cells, paraspeckles are discrete bodies in the interchromatin nucleoplasmic space which contain p54/nrb (nuclear RNA-binding protein) and at least two other RNA-binding proteins, paraspeckle protein 1 (PSP1) and paraspeckle protein 2 (PSP2). Paraspeckles often co-localize with splicing speckles, which are the site of splicing factor accumulation. Paraspeckle proteins, including p54/nrb, move dynamically between the nucleolus and paraspeckles and translocate to distinct caps in the nucleolar periphery when transcription is inhibited. Originally purified from HeLa cells, the nuclear p54/nrb has two RNA recognition motifs and shares extensive homology with both the human splicing factor PSF and Drosophila NONA/BJ6, which is required for normal vision and courtship. The shared domain between these proteins is termed a DBHS (Drosophila behavior, human splicing) domain and may play a role in regulating various pathways at the level of pre-mRNA splicing. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: C-terminal human p54nrb |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 54 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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