Anti-Nociceptin receptor Polyclonal Antibody 2 is a Rabbit antibody targeting Nociceptin receptor. Anti-Nociceptin receptor Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,GuineaPig)

1. Sample:
HearT (Rat) lysate at 30 μg;
Brain (Rat) lysate at 30 μg;
Primary: Anti-Nociceptin receptor (TMAB-09551) at 1: 200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 40 kD
Observed band size: 40 kD
2. Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated (TMAB-09551) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated used at 1: 200 dilution for 40 minutes at 37°C.
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated (TMAB-09551) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-Nociceptin receptor (TMAB-09551) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 52 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Nociceptin receptor

Human

OPRL1

Nociceptin receptor

Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.