Anti-Nociceptin Polyclonal Antibody is a Rabbit antibody targeting Nociceptin. Anti-Nociceptin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Rat (predicted:Human,Mouse,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Nociceptin Polyclonal Antibody, Unconjugated (TMAB-09550) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Brain (Rat) lysate at 30 μg;
liver (Rat) lysate at 30 μg;
Primary: Anti-Nociceptin (TMAB-09550) at 1: 200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size: 20 kD
Observed band size: 20 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Nociceptin

Human

PNOC

Prepronociceptin

Nociceptin is the ligand of the opioid receptor-like receptor (OPRL1). It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development (By similarity).