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Anti-nNOS Polyclonal Antibody
😃Good
Catalog No. TMAB-01250
Anti-nNOS Polyclonal Antibody is a Rabbit antibody targeting nNOS. Anti-nNOS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-nNOS Polyclonal Antibody
Anti-nNOS Polyclonal Antibody
😃Good
Catalog No. TMAB-01250
Anti-nNOS Polyclonal Antibody is a Rabbit antibody targeting nNOS. Anti-nNOS Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg RAW246.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-nNOS (TMAB-01250) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 2. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-nNOS (TMAB-01250) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (nNOS) Polyclonal Antibody, Unconjugated (TMAB-01250) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-nNOS (TMAB-01250) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 150 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]. |
| Immunogen | KLH conjugated synthetic peptide: human nNos/NOS-1 |
| Antigen Species | Human |
| Gene ID | 4842 |
| Uniprot ID | |
| Biology Area | Germ cell markers,Regulation,Embryonic Germ Cells |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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