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Anti-nNOS Polyclonal Antibody
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Catalog No. TMAB-01250
Alias Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS
Anti-nNOS Polyclonal Antibody is a Rabbit antibody targeting nNOS. Anti-nNOS Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
Anti-nNOS Polyclonal Antibody
Anti-nNOS Polyclonal Antibody
😃Good
Catalog No. TMAB-01250Alias Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS
Anti-nNOS Polyclonal Antibody is a Rabbit antibody targeting nNOS. Anti-nNOS Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-nNOS Polyclonal Antibody is a Rabbit antibody targeting nNOS. Anti-nNOS Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB. |
| Synonyms | Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Sheep) |
| Verified Activity | 1. Sample: Hela (Human) Cell Lysate at 30 μg MCF-7 (Human) Cell Lysate at 30 μg A549 (Human) Cell Lysate at 30 μg RAW246.7 (Mouse) Cell Lysate at 30 μg Primary: Anti-nNOS (TMAB-01250) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 2. Sample: Testis (Mouse) Lysate at 40 μg Primary: Anti-nNOS (TMAB-01250) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 130 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (nNOS) Polyclonal Antibody, Unconjugated (TMAB-01250) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 4. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-nNOS (TMAB-01250) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kDa Observed band size: 150 kDa     |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). |
| Tissue Specificity | Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]. |
| Immunogen | KLH conjugated synthetic peptide: human nNos/NOS-1 |
| Antigen Species | Human |
| Gene Name | NOS1 |
| Gene ID | |
| Protein Name | Nitric oxide synthase, brain |
| Uniprot ID | |
| Biology Area | Germ cell markers,Regulation,Embryonic Germ Cells |
| Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. |
| Molecular Weight | Theoretical: 130 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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