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Anti-nNOS Antibody (1J290)
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Catalog No. TMAC-02869
Alias Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS
Anti-nNOS Antibody (1J290) is a Rabbit antibody targeting nNOS. Anti-nNOS Antibody (1J290) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-nNOS Antibody (1J290)
Anti-nNOS Antibody (1J290)
😃Good
Catalog No. TMAC-02869Alias Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS
Anti-nNOS Antibody (1J290) is a Rabbit antibody targeting nNOS. Anti-nNOS Antibody (1J290) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Chemical Properties
| Description | Anti-nNOS Antibody (1J290) is a Rabbit antibody targeting nNOS. Anti-nNOS Antibody (1J290) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Peptidyl-cysteine S-nitrosylase NOS1, NOS1, NOS type I, nNOS), N-NOS, Nitric oxide synthase, brain, Neuronal NOS(N-NOS, neuronal Nitric Oxide Synthase, NC-NOS, EC:1.14.13.39, Constitutive NOS, bNOS |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of nNOS on mouse heart lysates using anti-nNOS antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-nNOS antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-nNOS antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-nNOS antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-nNOS antibody. Counter stained with hematoxylin. 6. ICC staining nNOS in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining nNOS in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining nNOS in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of PC-12 cells with nNOS antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 161 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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