Anti-Nm23-H2/NME2 Antibody (9O567) is a Rabbit antibody targeting Nm23-H2/NME2. Anti-Nm23-H2/NME2 Antibody (9O567) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

9O567

Human,Mouse,Rat

1. Western blot analysis of LnCaP cell lysate. Using Nm23-H2/NME2 (TMAB-09524) monoclonal antibody at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The 293T (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Nm23-H2/NME2 antibody (TMAB-09524, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:1000-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100

Monoclonal

A synthesized peptide: human NDK B

Human

NME2

Nucleoside diphosphate kinase B

Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.