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Anti-NLRP3 Polyclonal Antibody is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-NLRP3 Polyclonal Antibody is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Chronic inflammatory mastitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. RAW264.7 (M) cells were treated with or without LPS (10 µg/ml) for 8h, 25 μg total protein per Lane of cell lysates probed with NALP3/CIAS1 polyclonal antibody, unconjugated (TMAB-01243) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 9. 25 μg total protein per Lane of various lysates probed with NALP3/CIAS1 polyclonal antibody, unconjugated (TMAB-01243) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Subcellular Location : Cytoplasm. |
Immunogen | KLH conjugated synthetic peptide: human Cryopyrin |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | TNF,Inflammatory mediators,Macrophage / Inflamm.,NFkB Pathway |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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