Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-NLRP3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01243

Anti-NLRP3 Polyclonal Antibody is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NLRP3 Polyclonal Antibody

Anti-NLRP3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01243
Anti-NLRP3 Polyclonal Antibody is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$374 7-10 days
200 μL$529 7-10 days
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-NLRP3 Polyclonal Antibody is a Rabbit antibody targeting NLRP3. Anti-NLRP3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Chronic inflammatory mastitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NALP3/CIAS1 Polyclonal Antibody, Unconjugated (TMAB-01243) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
8. RAW264.7 (M) cells were treated with or without LPS (10 µg/ml) for 8h, 25 μg total protein per Lane of cell lysates probed with NALP3/CIAS1 polyclonal antibody, unconjugated (TMAB-01243) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
9. 25 μg total protein per Lane of various lysates probed with NALP3/CIAS1 polyclonal antibody, unconjugated (TMAB-01243) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundMay function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Subcellular Location : Cytoplasm.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Cryopyrin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
TNF,Inflammatory mediators,Macrophage / Inflamm.,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: buy Anti-NLRP3 Polyclonal Antibody | purchase Anti-NLRP3 Polyclonal Antibody | Anti-NLRP3 Polyclonal Antibody cost | order Anti-NLRP3 Polyclonal Antibody