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Anti-Nkx2.5 Polyclonal Antibody
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Catalog No. TMAB-09507
Anti-Nkx2.5 Polyclonal Antibody is a Rabbit antibody targeting Nkx2.5. Anti-Nkx2.5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Nkx2.5 Polyclonal Antibody
Anti-Nkx2.5 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-09507
Anti-Nkx2.5 Polyclonal Antibody is a Rabbit antibody targeting Nkx2.5. Anti-Nkx2.5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $374 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-Nkx2.5 Polyclonal Antibody is a Rabbit antibody targeting Nkx2.5. Anti-Nkx2.5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit) |
| Verified Activity | 1. Tissue/cell: Rat heart; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Nkx2.5/Cardiac-specific homeobox 1 Polyclonal Antibody, Unconjugated (TMAB-09507) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Nkx2.5) Polyclonal Antibody, Unconjugated (TMAB-09507) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nuclear |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | In humans, mutations of the gene encoding the homeobox transcription factor Nkx2.5 result in electrical conduction defects and morphological abnormalities of the heart (Dupays et al., 2005). In the heart, Nkx2.5 is expressed in both the myocardium and the endocardium. Differentiation of embryonic stem cells to Nkx2.5-positive cardiomyocytes is facilitated by Wnt11. |
| Immunogen | KLH conjugated synthetic peptide: human Nkx2.5 |
| Antigen Species | Human |
| Gene Name | NKX2-5 |
| Gene ID | |
| Protein Name | Homeobox protein Nkx-2.5 |
| Uniprot ID |
| Molecular Weight | Theoretical: 36 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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