Anti-NKG2D Polyclonal Antibody 3 is a Rabbit antibody targeting NKG2D. Anti-NKG2D Polyclonal Antibody 3 can be used in WB,FCM.

IgG

Mouse (predicted:Human,Rat,Pig)

1. Sample: Spleen (Mouse) Lysate at 40 μg
Primary: Anti-NKG2D (TMAB-09497) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 26 kD
2. Blank control (blue line): Mouse thymus cells (fixed with 70% methanol (Overnight at 4℃). Cells stained with Primary Antibody for 30 min at room temperature. The cells).
Primary Antibody (green line): Rabbit Anti-NKG2D antibody (TMAB-09497), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.
3. Sample:
Lung (Mouse) Lysate at 40 μg
Raw246.7 (Mouse) Cell Lysate at 40 μg
Spleen (Mouse) Lysate at 40 μg
Lymph node (Mouse) Lysate at 40 μg
Bone (Rat) Lysate at 40 μg
Primary: Anti-NKG2D (TMAB-09497) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human NKG2D

Human

KLRK1

NKG2-D type II integral membrane protein

Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.