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Anti-Nicastrin Antibody (6Q347)
😃Good
Catalog No. TMAY-01722
Alias nicastrin, Nct, NCSTN, mKIAA0253, Kiaa0253, D1Dau13e, Aph2, AA727311, 9430068N19Rik
Anti-Nicastrin Antibody (6Q347) is a Rabbit antibody targeting Nicastrin. Anti-Nicastrin Antibody (6Q347) can be used in WB,IP.
Anti-Nicastrin Antibody (6Q347)
Anti-Nicastrin Antibody (6Q347)
😃Good
Catalog No. TMAY-01722Alias nicastrin, Nct, NCSTN, mKIAA0253, Kiaa0253, D1Dau13e, Aph2, AA727311, 9430068N19Rik
Anti-Nicastrin Antibody (6Q347) is a Rabbit antibody targeting Nicastrin. Anti-Nicastrin Antibody (6Q347) can be used in WB,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, IP Reactivity: Human |
| Alias | nicastrin, Nct, NCSTN, mKIAA0253, Kiaa0253, D1Dau13e, Aph2, AA727311, 9430068N19Rik |
| Ig Type | Rabbit IgG |
| Clone | 6Q347 |
| Reactivity | Human |
| Verified Activity | 1. Anti-NCSTN rabbit monoclonal antibody at 1:500 dilution. -Lane A: Jurkat Whole Cell Lysate. -Lane B: A431 Whole Cell Lysate. -Lane C: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:78 kDa. -Observed band size:170 kDa(We are unsure as to the identity of these extra bands.) 2. NCSTN was immunoprecipitated using: -Lane A:0.5 mg Jurkat Whole Cell Lysate. -Lane B:0.5 mg 293T Whole Cell Lysate -0.5 µL anti-NCSTN rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-NCSTN rabbit monoclonal antibody, at 1:150 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 78 kDa. -Observed band size: 130 kDa |
| Application | WB,IP |
| Recommended Dose | WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin / NCSTN (rh Nicastrin / NCSTN; TMPY-01297; NP_056146.1; Met1-Glu669). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing pieces of evidence have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex that also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of another type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). Besides, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Nicastrin / NCSTN protein (TMPY-01297) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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