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Anti-NHEJ1 Antibody (5C811)

Catalog No. TMAB-09466 Copy Product Info
🥰Excellent
Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting . Anti-NHEJ1 Antibody (5C811) can be used in WB,FCM.

Anti-NHEJ1 Antibody (5C811)

Copy Product Info
🥰Excellent
Catalog No. TMAB-09466

Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting . Anti-NHEJ1 Antibody (5C811) can be used in WB,FCM.

Anti-NHEJ1 Antibody
(5C811)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1497-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting . Anti-NHEJ1 Antibody (5C811) can be used in WB,FCM.
Ig Type
IgG
Clone
5C811
Reactivity
Human
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with NHEJ1 monoclonal antibody, unconjugated (TMAB-09466) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NHEJ1 antibody (TMAB-09466, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB=1:500-2000,FCM=1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundDNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination.Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends.Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends.
Antigen Details
Immunogen
A synthesized peptide: human NHEJ1
Antigen Species
Human
Gene Name
NHEJ1
Gene ID
Protein Name
Non-homologous end-joining factor 1
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 33 kDa. Actual: 39 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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