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Anti-NHEJ1 Antibody (5C811)
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Catalog No. TMAB-09466
Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting NHEJ1. Anti-NHEJ1 Antibody (5C811) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-NHEJ1 Antibody (5C811)
Anti-NHEJ1 Antibody (5C811)
Copy Product Info😃Good
Catalog No. TMAB-09466
Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting NHEJ1. Anti-NHEJ1 Antibody (5C811) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $149 | 7-10 days | 7-10 days | |
| 50 μL | $263 | 7-10 days | 7-10 days | |
| 100 μL | $473 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-NHEJ1 Antibody (5C811) is a Rabbit antibody targeting NHEJ1. Anti-NHEJ1 Antibody (5C811) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
| Ig Type | IgG |
| Clone | 5C811 |
| Reactivity | Human (predicted:Mouse,Rat) |
| Verified Activity | 1. 25 ug total protein per lane of various lysates (see on figure) probed with NHEJ1 monoclonal antibody, unconjugated (TMAB-09466) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-NHEJ1 antibody (TMAB-09466, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination.Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends.Together with PAXX, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends. |
| Immunogen | A synthesized peptide: human NHEJ1 |
| Antigen Species | Human |
| Gene Name | NHEJ1 |
| Gene ID | |
| Protein Name | Non-homologous end-joining factor 1 |
| Uniprot ID |
| Molecular Weight | Theoretical: 33 kDa. Actual: 39 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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