Anti-NGFR/p75NTR Polyclonal Antibody 2 is a Rabbit antibody targeting NGFR/p75NTR. Anti-NGFR/p75NTR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p75 NGF Receptor) Polyclonal Antibody, Unconjugated (TMAB-09461) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p75 NGF Receptor) Polyclonal Antibody, Unconjugated (TMAB-09461) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NGFR Polyclonal Antibody, Unconjugated (TMAB-09461) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Blank control (blue line): Mouse spleen (fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (TMAB-09461), Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg /test.
5. Sample:
Lane 1: Recombinant human NGFR protein, C-His (HEK293)
Primary: Anti-NGFR/p75NTR (TMAB-09461) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 37 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human NGFR/p75NTR

Human

NGFR

Tumor necrosis factor receptor superfamily member 16

Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.