Anti-NGFR/p75NTR Polyclonal Antibody is a Rabbit antibody targeting NGFR/p75NTR. Anti-NGFR/p75NTR Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,GuineaPig)

1. Blank control: (mo) nephrocyte (blue)
Isotype Control Antibody: Rabbit IgG -AF647 (orange);
Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA (green).
2. Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (TMAB-09462)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1 μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ and then permeabilized with 0.1% P then permeabilized with 90% ice-cold methanol for 30 min on ice.). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
3. Blank control: Mouse brain.
Primary Antibody (green line): Rabbit Anti-p75 NGF Receptor antibody (TMAB-09462)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human NGFR

Human

NGFR

Tumor necrosis factor receptor superfamily member 16

Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.