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Anti-NFKB1 Antibody (6X277)
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Catalog No. TMAC-02821
Alias Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1, EBP-1, DNA-binding factor KBF1
Anti-NFKB1 Antibody (6X277) is a Mouse antibody targeting NFKB1. Anti-NFKB1 Antibody (6X277) can be used in WB,ICC,IHC.
Anti-NFKB1 Antibody (6X277)
Anti-NFKB1 Antibody (6X277)
😃Good
Catalog No. TMAC-02821Alias Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1, EBP-1, DNA-binding factor KBF1
Anti-NFKB1 Antibody (6X277) is a Mouse antibody targeting NFKB1. Anti-NFKB1 Antibody (6X277) can be used in WB,ICC,IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $426 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-NFKB1 Antibody (6X277) is a Mouse antibody targeting NFKB1. Anti-NFKB1 Antibody (6X277) can be used in WB,ICC,IHC. |
| Synonyms | Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p105 subunit, NFKB1, EBP-1, DNA-binding factor KBF1 |
| Clone | 6X277 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of NF-κB p105/p50 on MCF-7 cell lysates. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 antibody. Counter stained with hematoxylin. 3. ICC staining NF-κB p105/p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:200-500; ICC: 1:200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: NF-κB p105/p50 |
| Uniprot ID |
| Molecular Weight | Theoretical: 50 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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