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Anti-NFAT2 Polyclonal Antibody

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Catalog No. TMAB-01223
Alias Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448

Anti-NFAT2 Polyclonal Antibody is a Rabbit antibody targeting NFAT2. Anti-NFAT2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-NFAT2 Polyclonal Antibody

Anti-NFAT2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01223Alias Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448
Anti-NFAT2 Polyclonal Antibody is a Rabbit antibody targeting NFAT2. Anti-NFAT2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NFAT2 Polyclonal Antibody is a Rabbit antibody targeting NFAT2. Anti-NFAT2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
AliasNuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse)
Verified Activity
1. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-NFAT2 antibody (TMAB-01223)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NFAT2) Polyclonal Antibody, Unconjugated (TMAB-01223) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-NFAT2 antibody (TMAB-01223)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NFATc1
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Transcription factors/regulators,NFATs,Other factors,Non-CD,NFATS
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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