Anti-NF-L Antibody (9L974) is a Rabbit antibody targeting NF-L. Anti-NF-L Antibody (9L974) can be used in FCM,ICC/IF.

IgG

9L974

Human (predicted:Mouse,Rat)

1. The SH-SY5Y (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-NF-L antibody (TMAB-09449): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (NF-L) monoclonal Antibody, unconjugated (TMAB-09449) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM: 1μg/Test; ICC/IF: 1:50-200

Monoclonal

Recombinant Protein: human NF-L protein

Human

NEFL

Neurofilament light polypeptide

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.