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Anti-NF-L Antibody (4X503)
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Catalog No. TMAB-09445
Anti-NF-L Antibody (4X503) is a Mouse antibody targeting NF-L. Anti-NF-L Antibody (4X503) can be used in IF, IHC-Fr, IHC-P, WB.
Anti-NF-L Antibody (4X503)
Anti-NF-L Antibody (4X503)
Copy Product Info😃Good
Catalog No. TMAB-09445
Anti-NF-L Antibody (4X503) is a Mouse antibody targeting NF-L. Anti-NF-L Antibody (4X503) can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-NF-L Antibody (4X503) is a Mouse antibody targeting NF-L. Anti-NF-L Antibody (4X503) can be used in IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG2a |
| Clone | 4X503 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Lane 1: Human U251 cell lysates Primary: Anti-NF-L (TMAB-09445) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 68 kDa 2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (TMAB-09445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (TMAB-09445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (TMAB-09445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IF=1:200-500; IHC-Fr=1:200-500; IHC-P=1:200-500; WB=1:500-2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M PBS (pH7.4). |
| Research Background | Neurofilament light polypeptide also called NF-L; Neurofilament triplet L protein; 68 kDa neurofilament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions. NF-L is the most abundant of the three neurofilament proteins and, as the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments. Belongs to the intermediate filament family. |
| Immunogen | Recombinant Protein: Human NF-L Protein |
| Antigen Species | Human |
| Gene Name | NEFL |
| Gene ID | |
| Protein Name | Neurofilament light polypeptide |
| Uniprot ID | |
| Function | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
| Molecular Weight | Theoretical: 68 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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