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Anti-NF-kB p65 Antibody (5J938)

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Catalog No. TMAB-01227
Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3

Anti-NF-kB p65 Antibody (5J938) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (5J938) can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NF-kB p65 Antibody (5J938)

Anti-NF-kB p65 Antibody (5J938)

😃Good
Catalog No. TMAB-01227Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Anti-NF-kB p65 Antibody (5J938) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (5J938) can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-NF-kB p65 Antibody (5J938) is a Mouse antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (5J938) can be used in WB,IHC-P,IHC-Fr,IF.
Aliasv-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Ig Type
IgG1
Clone
5J938
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 μg total protein per Lane of various lysates probed with NFKB p65 monoclonal antibody, unconjugated (TMAB-01227) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01227) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01227) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01227) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of TMAB-01227) at 1:1500 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:200-1500; IHC-Fr: 1:200-1500; IF: 1:200-1500
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus. Cytoplasm. Note=Colocalized with DDX1 in the nucleus upon TNF-alpha induction. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NFKB p65
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
RelA (p65),NFkB pathway,Inflammatory mediators,RelA (p65),NFkB pathway,Obesity,Host Immune Response,Host Virus Interaction,Alzheimer's disease,NFkB Pathway
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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