Anti-Neutrophil Elastase Polyclonal Antibody is a Rabbit antibody targeting Neutrophil Elastase. Anti-Neutrophil Elastase Polyclonal Antibody can be used in WB,ICC/IF,FCM.

IgG

Human

1. 25 μg total protein per lane of various lysates (see on figure) probed with Neutrophil Elastase polyclonal antibody, unconjugated (TMAB-09419) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed THP-1 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Neutrophil Elastase) polyclonal Antibody, unconjugated (TMAB-09419) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
3. The THP-1 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Neutrophil Elastase antibody (TMAB-09419): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC: 1 μg/test. Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.

WB: 1:500-2000; ICC/IF: 1:50-200; FCM: 1μg/Test

Polyclonal

Recombinant Protein: human Neutrophil Elastase protein

Human

ELANE

Neutrophil elastase

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis.