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Anti-Neurotrophin 4 Polyclonal Antibody is a Rabbit antibody targeting Neurotrophin 4. Anti-Neurotrophin 4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
| Description | Anti-Neurotrophin 4 Polyclonal Antibody is a Rabbit antibody targeting Neurotrophin 4. Anti-Neurotrophin 4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Neurotrophin 4/NT-4/NT-5 Polyclonal Antibody, Unconjugated (TMAB-09416) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: DU145 Cell (Human) Lysate at 40 μg Primary: Anti-Neurotrophin 4 (TMAB-09416) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 14/27 kD Observed band size: 26 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Tissue Specificity | Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Neurotrophin 4 (NT4; synonymous with NT5) belongs to the NGF beta family and is a survival factor for peripheral sensory sympathetic neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. NT4 levels are highest in the prostate, with lower levels in thymus, placenta, and skeletal muscle. NT4 is also expressed in embryonic and adult tissues. |
| Immunogen | KLH conjugated synthetic peptide: human NT-4 |
| Antigen Species | Human |
| Gene Name | NTF4 |
| Gene ID | |
| Protein Name | Neurotrophin-4 |
| Uniprot ID | |
| Function | Target-derived survival factor for peripheral sensory sympathetic neurons. |
| Molecular Weight | Theoretical: 14/27 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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