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Anti-Neuronatin Polyclonal Antibody
Catalog No. TMAB-09400 Copy Product Info
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Anti-Neuronatin Polyclonal Antibody is a Rabbit antibody targeting Neuronatin. Anti-Neuronatin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Neuronatin Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09400
Anti-Neuronatin Polyclonal Antibody is a Rabbit antibody targeting Neuronatin. Anti-Neuronatin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Neuronatin Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Bioactivity
Antigen Details
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| Description | Anti-Neuronatin Polyclonal Antibody is a Rabbit antibody targeting Neuronatin. Anti-Neuronatin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Rat (predicted:Human,Mouse,Pig,Cow,Rabbit) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Neuronatin) Polyclonal Antibody, Unconjugated (TMAB-09400) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The paternally imprinted Neuronatin gene (NNAT) is initially expressed in rhombomeres and the pituitary gland and is later expressed more widely, but much less abundantly, in the central and peripheral nervous systems. The human NNAT gene maps to chromosome 20q11.23 and contains an imprinting region associated with morphological abnormalities and early neonatal lethality. Specifically, hypermethylation of the NNAT gene occurs in both myeloid and lymphoid acute pediatric leukemias and may inhibit NNAT expression. The Neuronatin protein consists of two isoforms, alpha and beta, which are the products of alternative splicing. The alpha form of the Neuronatin gene is encoded by three exons, whereas the beta form is missing the second exon. Neuronatin mRNA expression is abundant in undifferentiated PC-12 cells. Treatment of these cells with nerve growth factor (NGF), which contributes to neuronal differentiation, downregulates Neuronatin mRNA expression. NNAT (-) 1.9 PC-12 cells exhibit an increase in nigericin, rotenone and valinomcin sensitivity; NNAT transfection restores wild-type PC-12 resistance. These results suggests a potential protective role for Neuronatin against toxic insult during development. |
| Immunogen | KLH conjugated synthetic peptide: human Neuronatin |
| Antigen Species | Human |
| Gene Name | NNAT |
| Gene ID | |
| Protein Name | Neuronatin |
| Uniprot ID | |
| Function | May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels. |
| Molecular Weight | Theoretical: 9 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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