Anti-Neuroligin 1 Polyclonal Antibody is a Rabbit antibody targeting Neuroligin 1. Anti-Neuroligin 1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-Neuroligin 1 (TMAB-09398) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
2. Blank control (blue): Mouse brain Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-Neuroligin 1 /PE Conjugated antibody (TMAB-09398 /PE), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/Pe (orange), used under the same conditions.
3. Blank control (blue line): U251 (fixed with 70% ethanol for 5 min at -20℃).
Primary Antibody (green line): Rabbit Anti-Neuroligin 1 antibody (TMAB-09398),
Dilution: 0.2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 μg /test.
4. Sample: Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-Neuroligin 1 (TMAB-09398) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD
5. Sample: Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Neuroligin 1 (TMAB-09398) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 87 kD
Observed band size: 87 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Neuroligin 1

Human

NLGN1

Neuroligin-1

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses.