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Anti-Neurofascin Polyclonal Antibody
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Catalog No. TMAB-09388
Anti-Neurofascin Polyclonal Antibody is a Rabbit antibody targeting Neurofascin. Anti-Neurofascin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Neurofascin Polyclonal Antibody
Anti-Neurofascin Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-09388
Anti-Neurofascin Polyclonal Antibody is a Rabbit antibody targeting Neurofascin. Anti-Neurofascin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Neurofascin Polyclonal Antibody is a Rabbit antibody targeting Neurofascin. Anti-Neurofascin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Horse) |
| Verified Activity | 1. Tissue/cell: rat spinal cord tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated (TMAB-09388) 1: 200, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining 2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Neurofascin Polyclonal Antibody, Unconjugated (TMAB-09388) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody and DAB staining 3. Sample: Cerebellum (Mouse) Lysate at 30 μg Primary: Anti-Neurofascin Polyclonal (TMAB-09388) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132/150 kD Observed band size: 136 kD 4. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: Cerebellum (Mouse) Lysate at 40 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: Cerebellum (Rat) Lysate at 40 μg Primary: Anti-Neurofascin (TMAB-09388) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132 kD Observed band size: 132 kD 5. Sample: Cerebral cortex (Mouse) Lysate at 40 μg Primary: Anti-Neurofascin (TMAB-09388) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 132/150 kD Observed band size: 150 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Neurofascin is a cell adhesion molecule involved in mediating axon recognition but also signaling axonal contact. Immunoglobulin domain cell adhesion molecule (cam) subfamily; members are components of neural cell adhesion molecules (N-CAM L1), Fasciclin II and the insect immune protein Hemolin. The subfamily also includes receptor domains such as as the extracelluar ligand binding domain of Fibroblast Growth Factor Receptor 2. Members are phylogenetically diverse, occuring throughout metazoa, and are not components of the adaptive immune system molecules found in jawed vertebrates. A predominant feature of most Ig domains is a disulfide bridge connecting 2 beta-sheets with a Trp packing against the disulfide bond. |
| Immunogen | KLH conjugated synthetic peptide: human Neurofascin-155 |
| Antigen Species | Human |
| Gene Name | NFASC |
| Gene ID | |
| Protein Name | Neurofascin |
| Uniprot ID | |
| Function | Cell adhesion, ankyrin-binding protein which may be involved in neurite extension, axonal guidance, synaptogenesis, myelination and neuron-glial cell interactions. |
| Molecular Weight | Theoretical: 132/150 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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