Anti-Neurocan Antibody (9D35) is a Rabbit antibody targeting Neurocan. Anti-Neurocan Antibody (9D35) can be used in WB,IHC-P,IHC-Fr,IP,IF.

IgG

9D35

Human

1. Western blot analysis of Fetal brain cell lysate. Using Neurocan (TMAB-09384) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Neurocan Monoclonal Antibody, Unconjugated (TMAB-09384) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Neurocan Monoclonal Antibody, Unconjugated (TMAB-09384) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.

WB: 1:1000-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IP: 1:20-50; IF: 1:100-500

Monoclonal

A synthesized peptide: human Neurocan

Human

NCAN

Neurocan core protein

May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid.