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Anti-NeuN Antibody (8Z879)

😃Good
Catalog No. TMAC-02782
Alias RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C

Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in WB,ICC/IF,IHC,FCM.

Anti-NeuN Antibody (8Z879)

Anti-NeuN Antibody (8Z879)

😃Good
Catalog No. TMAC-02782Alias RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in WB,ICC/IF,IHC,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4967-10 days
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Product Introduction

Bioactivity
Description
Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in WB,ICC/IF,IHC,FCM.
SynonymsRNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Ig Type
IgG
Clone
8Z879
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa).
2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin.
4. ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundNeuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 34 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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