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Anti-NeuN Antibody (8Z879)
😃Good
Catalog No. TMAC-02782
Alias RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in FCM,ICC/IF,IHC,WB.
Anti-NeuN Antibody (8Z879)
Anti-NeuN Antibody (8Z879)
😃Good
Catalog No. TMAC-02782Alias RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C
Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Chemical Properties
| Description | Anti-NeuN Antibody (8Z879) is a Rabbit antibody targeting NeuN. Anti-NeuN Antibody (8Z879) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | RNA binding protein fox-1 homolog 3, RFOX3, RBFOX3, Neuronal nuclei antigen(NeuN antigen), HRNBP3, FOX-3, FOX3, Fox-1 homolog C |
| Ig Type | IgG |
| Clone | 8Z879 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of NeuN on human brain lysates using anti-NeuN antibody at 1/1,000 dilution. (NeuN is expressed as two alternate transcripts with apparent SDS-PAGE molecular weight 46 and 48kDa). 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NeuN antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-NeuN antibody. Counter stained with hematoxylin. 4. ICC staining NeuN in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of SH-SY-5Y cells with NeuN antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.      |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:200-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 34 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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