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Anti-NeuN Antibody (4I590)

Catalog No. TMAB-09372 Copy Product Info
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Anti-NeuN Antibody (4I590) is a Mouse antibody targeting NeuN. Anti-NeuN Antibody (4I590) can be used in IHC-P,IHC-Fr,IF.

Anti-NeuN Antibody (4I590)

Copy Product Info
🥰Excellent
Catalog No. TMAB-09372

Anti-NeuN Antibody (4I590) is a Mouse antibody targeting NeuN. Anti-NeuN Antibody (4I590) can be used in IHC-P,IHC-Fr,IF.

Anti-NeuN Antibody
(4I590)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1487-10 days7-10 days
50 μL$2637-10 days7-10 days
100 μL$4727-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-NeuN Antibody (4I590) is a Mouse antibody targeting NeuN. Anti-NeuN Antibody (4I590) can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Clone
4I590
Reactivity
Mouse,Rat (predicted:Human)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NeuN) Monoclonal Antibody, Unconjugated (TMAB-09372) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NeuN) Monoclonal Antibody, Unconjugated (TMAB-09372) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NeuN) Monoclonal Antibody, Unconjugated (TMAB-09372) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (NeuN) Monoclonal Antibody, Unconjugated (TMAB-09372) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
Application
Recommended Dose
IHC-P: 1:50-200; IHC-Fr: 1:50-200; IF: 1:50-200
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationNucleus. Cytoplasm.
Tissue SpecificityExpressed in activated, but not resting, CD4+ T-cells and activated monocytes.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
Antigen Details
Immunogen
Recombinant Protein: human NeuN
Antigen Species
Human
Gene Name
RBFOX3
Gene ID
Protein Name
RNA binding protein fox-1 homolog 3
Uniprot ID
Function
RNA-binding protein that regulates alternative splicing events.
Chemical Properties
Molecular WeightTheoretical: 34 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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