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Anti-Nestin/NES Polyclonal Antibody
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Catalog No. TMAB-01215
Alias Nestin, NEST_RAT, Nes, Nbla00170, Intermediate filament protein, FLJ21841, FLJ 21841, ESTM 46
Anti-Nestin/NES Polyclonal Antibody is a Rabbit antibody targeting Nestin/NES. Anti-Nestin/NES Polyclonal Antibody can be used in FCM.
Anti-Nestin/NES Polyclonal Antibody
Anti-Nestin/NES Polyclonal Antibody
😃Good
Catalog No. TMAB-01215Alias Nestin, NEST_RAT, Nes, Nbla00170, Intermediate filament protein, FLJ21841, FLJ 21841, ESTM 46
Anti-Nestin/NES Polyclonal Antibody is a Rabbit antibody targeting Nestin/NES. Anti-Nestin/NES Polyclonal Antibody can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Nestin/NES Polyclonal Antibody is a Rabbit antibody targeting Nestin/NES. Anti-Nestin/NES Polyclonal Antibody can be used in FCM. |
| Synonyms | Nestin, NEST_RAT, Nes, Nbla00170, Intermediate filament protein, FLJ21841, FLJ 21841, ESTM 46 |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Rat) |
| Verified Activity | Cell: Neuro-2a Concentration:1:100 Host/Isotype: Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a (blue) compared with primary antibody (Cat#: TMAB-01215)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit Igg (H+L) secondary antibody.  |
| Application | |
| Recommended Dose | FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | cytoplasm |
| Tissue Specificity | CNS stem cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. |
| Immunogen | KLH conjugated synthetic peptide: rat Nestin |
| Antigen Species | Rat |
| Gene Name | NES |
| Gene ID | |
| Protein Name | Nestin |
| Uniprot ID | |
| Biology Area | Angiogenesis,Tumor antigens,Angiogenic Factors,Ectoderm,Intermediate Filaments,Neural Stem Cell marker,Neurogenesis,nestin,Ectoderm,Intracellular,Neuron Restricted Lineage |
| Function | Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Molecular Weight | Theoretical: 209 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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