Antibody Type: Mouse Monoclonal

Application: WB, IHC-P, FCM, IP

Reactivity: Human

Mouse IgG2a

1C457

Human

1. Immunochemical staining of human Nestin in human brain with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
2. Immunochemical staining of human Nestin in human neurilemmoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
3. Immunochemical staining of human Nestin in human fasciculated sarcoma with mouse monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
4. Flow cytometric analysis of Human NES expression on SHSY5Y cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human NES, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
5. Anti-NES mouse monoclonal antibody at 1:500 dilution.
-Lane A: HeLa Whole Cell Lysate.
-Lane B: U251MG Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:177 kDa.
6. NES was immunoprecipitated using:
-Lane A:0.5 mg U-251 MG Whole Cell Lysate.
-4 µL anti-NES mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-NES mouse monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 177 kDa.
-Observed band size:177 kDa

WB,IHC-P,FCM,IP

WB: 1:500-1:2000; IHC-P: 1:200-1:2000; FCM: 1:25-1:100; IP: 1-5 µL/mg of lysate

Monoclonal

Unconjugated

A synthetic peptide: N-terminus of the Human Nestin / NES

Human