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Synonyms:
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 μL | $199 | 7-10 days | 7-10 days |
| Description | Anti-Nestin/NES Antibody (1C457) is a Mouse antibody targeting Nestin/NES. Anti-Nestin/NES Antibody (1C457) can be used in FCM,IHC-P,IP,WB. |
| Ig Type | Mouse IgG2a |
| Clone | 1C457 |
| Reactivity | Human |
| Specificity | Human Nestin |
| Application | |
| Recommended Dose | WB: 1:500-1:2000; IHC-P: 1:200-1:2000; FCM: 1:25-1:100; IP: 1-5 µL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the Human Nestin / NES. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | NES (Nestin) is a Protein Coding gene. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in breast cancer (BC) and other cancer types. Nestin is an intermediate filament protein and participates in the cytoskeleton organization. Nestin expression in the endothelium of blood vessels is mainly limited to newly forming vessels, thus being a more specific marker of angiogenesis than the commonly used vascular antigens. It is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. Nestin is broadly expressed in the heart, fat, and other tissues. Diseases associated with NES include Ependymoblastoma and Periventricular Leukomalacia. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: N-terminus of the Human Nestin / NES |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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