Anti-Nestin Antibody (6Y635) is a Mouse antibody targeting Nestin. Anti-Nestin Antibody (6Y635) can be used in IHC-P,WB,IHC-Fr,IF.

IgG2a, κ

6Y635

Human

1. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (TMAB-09359) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (TMAB-09359) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Nestin) Monoclonal Antibody, Unconjugated (TMAB-09359) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (TMAB-09359) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
5. 25 μg total protein per lane of various lysates (see on figure) probed with Nestin monoclonal antibody, unconjugated (TMAB-09359) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

IHC-P: 1:100-500; WB: 1:500-1000; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Nestin

Human

NES

Nestin

Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.