Anti-NEK9 Polyclonal Antibody is a Rabbit antibody targeting NEK9. Anti-NEK9 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Tissue/cell: Mouse testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NEK9 Polyclonal Antibody, Unconjugated (TMAB-09350) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NEK9

Human

NEK9

Serine/threonine-protein kinase Nek9

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.