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Anti-NEK7 Polyclonal Antibody
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Catalog No. TMAB-01213
Alias NIMA-related kinase 7
Anti-NEK7 Polyclonal Antibody is a Rabbit antibody targeting NEK7. Anti-NEK7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-NEK7 Polyclonal Antibody
Anti-NEK7 Polyclonal Antibody
😃Good
Catalog No. TMAB-01213Alias NIMA-related kinase 7
Anti-NEK7 Polyclonal Antibody is a Rabbit antibody targeting NEK7. Anti-NEK7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-NEK7 Polyclonal Antibody is a Rabbit antibody targeting NEK7. Anti-NEK7 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | NIMA-related kinase 7 |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Pig,Cow,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated (TMAB-01213) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (rat thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated (TMAB-01213) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NEK7) Polyclonal Antibody, Unconjugated (TMAB-01213) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Tissue specificity: Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. |
| Immunogen | KLH conjugated synthetic peptide: human NEK7 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cytokinesis,Other cell division antibodies,Kinases/Phosphatases,Other Kinases |
| Molecular Weight | Theoretical: 35 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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