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Anti-NEK11 Polyclonal Antibody

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Catalog No. TMAB-09343

Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-NEK11 Polyclonal Antibody

Anti-NEK11 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-09343
Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2207-10 days7-10 days
100 μL$3737-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NEK11) Polyclonal Antibody, Unconjugated (TMAB-09343) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Sample: Hela (Human) Cell Lysate at 40 μg
Primary: Anti-NEK11 (TMAB-09343) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54'7 4 kD
Observed band size: 70 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.
Tissue SpecificityPoorly expressed in cerebellum, trachea, lung, appendix, and uterus.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human NEK11
Antigen Species
Human
Gene Name
NEK11
Gene ID
Protein Name
Serine/threonine-protein kinase Nek11
Uniprot ID
Function
Possible role in S-phase checkpoint mechanism.
Chemical Properties
Molecular WeightTheoretical: 54 and 74 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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