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Anti-NEK11 Polyclonal Antibody
Catalog No. TMAB-09343 Copy Product Info
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Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-NEK11 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-09343
Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-NEK11 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
Stability & Storage
| Description | Anti-NEK11 Polyclonal Antibody is a Rabbit antibody targeting NEK11. Anti-NEK11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (NEK11) Polyclonal Antibody, Unconjugated (TMAB-09343) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Hela (Human) Cell Lysate at 40 μg Primary: Anti-NEK11 (TMAB-09343) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54'7 4 kD Observed band size: 70 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. |
| Tissue Specificity | Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] |
| Immunogen | KLH conjugated synthetic peptide: human NEK11 |
| Antigen Species | Human |
| Gene Name | NEK11 |
| Gene ID | |
| Protein Name | Serine/threonine-protein kinase Nek11 |
| Uniprot ID | |
| Function | Possible role in S-phase checkpoint mechanism. |
| Molecular Weight | Theoretical: 54 and 74 kDa. Actual: 70 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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